In assisted reproductive technology (ART), cryopreservation of human oocytes and embryos has been significantly improved by a new method called vitrification. It is a great advancement over the slow – cooling method.
In The slow-cooling method, a programmed cryo-machine is needed and takes several hours. Still, it is difficult to eliminate injuries resulting from ice crystal formation completely.
Vitrification, on the other hand, is more reliable because it is simple, can lead to high recovery rates and viability (97%), and has better implantation and pregnancy rates.
Vitrification transforms cells into an amorphous glassy state with ultra-rapid cooling better cryoprotectants, smaller volume. The cooling and thawing steps by plunging the oocytes and embryos into liquid nitrogen. In the last 10 years, advancement in vitrification technologies has improved the clinical capabilities and their results
Freezing Embryos and Eggs
When patients undergo an in vitro fertilization (IVF) cycle, they will be several numbers of viable embryos. Not all the embryos are transferred, some can be vitrified and stored till needed to be transferred later.
Unfertilized eggs can also be frozen for the future use, whenever there would be requirement of unfertilized eggs for IVF treatment. They can be thawed and fertilized and then the healthy embryos can be transferred into the uterus.
Vitrification at Delhi IVF:
our lab uses vitrification in all freezing procedures currently performed. We are also happy to confirm numerous pregnancies that have resulted from both cryopreserved eggs and embryos using vitrification in our lab. Pregnancy and implantation rates become higher when embryos are transferred into a more receptive endometrium, without hormonal treatment.
All cryopreservation’s, sperms, oocytes an embryos are being vitrified in our laboratory for future use.