Endometrial thickness is an important factor in implantation and pregnancy. In several studies, the minimum endometrial thickness for embryo transfer has been reported to be 7 mm. Several approaches have been tried to increase endometrial thickness for frozen-thawed embryo transfer (FET) cyclesbut as yet there is no consensus on the most effective method. Some FET cycles are cancelled due to thin endometrium despite routine treatment, and there is no recognized protocol for increasing the thin endometrium.
Chang et.al. 2015 has proposed a new approach for the treatment of thin endometrium, by infusion of Platelet Rich Plasma (PRP).
Fresh PRP prepared from whole blood, collected from peripheral veins contains several growth factors such as:
PRP has been used in several other medical conditions in e.g. sports injuries, diabetic foot healing, orthopedics, surgery and wound healing but its efficacy in endometrial growth has not been fully elucidated.
Hysteroscopic examination is performed before the cycle if it had not been done previously. Hormone replacement therapy (HRT) is started on the day of the cycle for endometrial preparation for IVF cycle Transvaginal ultrasound is performed by an expert gynecologist by one machine. Endometrial thickness is measured at the thickest part in the longitudinal axis of the uterus.
PRP is prepared from patients own blood using a two-step centrifuge process.
On the 9th or 10th day of the menstrual cycle, the blood is processed as follows:
This procedure results in endometrial thickening, improved implantation rates and clinical pregnancies.
Patients suffering from chronic thin endometrium can benefit from PRP therapy. The successful implantation depends upon the thickness and close dialog between the blastocyst and the endometrium.
“Several methods are performed for endometrial preparation in frozen-thawed embryo transfer (FET) cycles, and there is little consensus on the most effective route.
“On the 9th or 10th day of the mensural cycle, 17.5 ml of peripheral venous blood is drawn in the syringe that containing 2.5 ml of Acid Citrate A Anticoagulant solution (ACD-A) and centrifuged immediately at 1200 rpm for 12 min to separate the red blood cells.”
“The plasma is centrifuged again at 3300 rpm for 7 min to obtain the PRP. Then, 0.5 ml of PRP is infused into the uterine cavity with the IUI catheter (as shown above).”
“The primary outcome is endometrial expansion and the secondary outcomes are chemical and clinical pregnancies, determined by positive serum βHCG, 2 weeks after ET.
Feeling very happy to have this type of experience. Just because of (IVF) we got twince babies in our life. Thanks you so much Dr. Anoop Gupta and his team. Thanks
First of all thanks to Delhi IVF centre & it’s all staff to bring happiest moment of our life. We feels proud every time we are here. One of the Best IVF centre I have visited ever. Staff is very co-operative. If someone will ask for IVF centre reference then definitely my answer will be Delhi IVF centre.
मेने डॉ. साहब का दिल से धन्यवाद करती हूँ क्यूंकि डॉ. साहब की कोशिश से हमारा सपना पूरा हुआ है | मुझे एक प्यारी सी बेटी की माँ बनने का सपना पूरा हुआ | मै बहुत खुश हूँ
We are so happy for the treatment of Dr. Gupta. We have so many problem in India before I tried many hospital for my wife and i found Delhi IVF. Thank you so much to the all team everyone was friendly with us. All the best for everyone.
I had the opportuned to see you early this year as regards my infertility problem and i am proud to inform you that i was delivered yesterday by C-section of a healthy 3.2 kg baby girl!!!! Many thanks to you, your lovely wife Dr. Alka and your dedicated management team. May God continue to bless you and the works of your hand.
Dear Dr. Anoop Gupta, & Team Greetings !!! Thanks You Once Again for changing our lives with your professional team and today we are living a happy and contented life as we got a gift with your efforts and medicines and our little Baby Girl Aaira was born on 20th February 2017 and our Family in now complete. My another friend Mr. Amin has shown his interest to visit your clinic and he wants to meet you and he has a great desire to have his treatment in your clinic.
Dear Dr. Aastha, Dr. Gupta and Team Hi from italy… I am so happy today Really want to thanks you. You’re so wonderful I had laparoscopy fibroid surgery few months ago. I went to see my doctor here in my country today and all my fibroids are gone. You have a done my surgery so sincerely you’re such a good human being can’t thank you enough I am so happy i had 2 surgeries earlier here in my city and both times I didn’t get better and it was the same. Now I am so happy May God Bless you
After long time my wishes fulfill. Thanks to Delhi IVF & Dr. Anoop Gupta & his team for giving me & my husband such a beautiful gift. Thank you very much.
Feeling very happy to have this type of experience. Just because of (IVF) we got twince babies in our life. Thanks you so much Dr. Anoop Gupta and his team. Thanks
Thank you to Dr. Anoop and his incredible team at Delhi IVF for bringing this bundle of joy to our family after a very long time.