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    Microscopic Testicular Sperm Extraction

    Microscopic testicular sperm extraction (microTESE) is a procedure that takes sperm directly from the testicular tissue of a man’s reproductive system. If a man can’t release or make enough healthy sperm naturally, this medical
    the procedure may be recommended for fertility reasons (to enable the man to father a child).
    In patients with non-obstructive azoospermia, micro the procedure increases the chances of sperm retrieval.

    What is microscopic testicular sperm extraction?

    Microscopic testicular sperm extraction (microTESE) is a procedure that takes sperm directly from the testicular tissue of a man’s reproductive system. If a man can’t release or make enough healthy sperm naturally, this
    the medical procedure may be recommended for fertility reasons (to enable the man to father a child).
    The testicular tissue is found in the two testes, where the sperm is made. The testes are found inside
    the scrotum, the small sac behind and under the penis.

    The goals of the microTESE procedure are to:

    • Obtain the best quality sperm.
    • Get enough sperm to fertilize an egg from a woman.
    • Minimize damage to the reproductive organs.

    What is male infertility?

    Infertility is a disease of the reproductive system that makes it difficult for the body to perform the
    basics of reproduction. It affects both men and women. Most infertility cases are treated with
    medication or surgery.
    Male infertility in its most severe form may be caused by a condition known as nonobstructive
    azoospermia (the man does not produce sperm), or obstructive azoospermia (sperm is produced,
    but blocked, and can’t be released from the body).
    Many times, the causes of infertility are unexplained. Sometimes, the difficulties may be from a
    genetic disease such as cystic fibrosis, a birth defect, a medical problem, or the result of an earlier
    treatment that may cause infertility (such as certain cancer treatments). In other cases, we do not
    know what the cause of the infertility is, but we still have treatments that may help.

    How is infertility diagnosed?

    If a couple is unable to conceive a child after several months of trying without birth control, they
    should see a doctor. If the woman is older than 35, the couple should see a doctor sooner. Your
    healthcare provider will perform a physical exam of both partners to check for any physical issues
    that may be causing infertility. The provider will also ask many questions and discuss each person’s
    medical history.

    The following tests may also be recommended or ordered to evaluate male fertility:

    • Semen analysis: Determines the number and quality of sperm. Semen is a bodily fluid that is
      secreted by the male reproductive organs. It carries sperm and other nutrients that help the
      sperm survive to enable successful fertilization.
    • Blood test: Checks for genetic or hormone problems. (Hormone levels are important in both
      male and female fertility.)
    • Ultrasound of the scrotum: Looks for abnormalities in the veins that carry blood from the
      testicles and back to the rest of the body.
    • In-home testing kits may be available for semen analysis. Ask the doctor for more information.

    What will the infertility tests determine?

    A semen analysis will give the doctor the necessary information to help assess fertility and create a
    treatment plan. The tests should show some of the following:

    • Amount of semen: At least 1.5 milliliters is considered normal. A lower number may mean
      that there is an internal issue with a part of the reproductive system, such as the seminal
      vesicles or a prostate gland, blocking the release of semen.
    • Sperm count: Fifteen million to 300 million per milliliter is considered a normal range. Below
      15 million is abnormal.
    • Morphology: The size and shape of the sperm. Four percent of normal-shaped sperm (using
      the “strict” criteria) is ideal to be able to fertilize an egg. Some doctors consider even a lower
      percentage as normal.
    • Motility: The movement of the sperm. Approximately 40% of sperm should be moving. The
      quality of movement is graded from 0 to 4, with a score over 3 considered good.

    When is microscopic testicular sperm extraction necessary or recommended?

    Microscopic testicular sperm extraction is usually performed on a man who is infertile and has no
    sperm in his ejaculation (azoospermia).

    What happens during the microscopic testicular sperm extraction procedure?

    A urologist, a doctor who specializes in the male and female urinary tract and male reproductive
    organs, typically performs the procedure by making a few small cuts in the testicle and removing a
    small amount of tissue. Once the tissue is removed from the body, it is examined under a microscope
    to look for sperm. If found, the sperm can be used right away to fertilize an egg, or the sperm cells
    can be frozen, stored, and then thawed at a later time to be used for infertility treatment.
    The procedure is usually performed in the operating room with the patient under general
    anesthesia. A single incision is made in the middle of the scrotum. A microscope is used to help
    see the fine tubules of the testis that may contain sperm. All sutures (stitches) that are used dissolve
    on their own. The patient is able to go home the day of the procedure and should be able to resume
    normal activities within one to two weeks.

    What are the possible complications of microscopic testicular sperm extraction?

    Possible risks of the microTESE procedure include bleeding, infection, and pain or discomfort after
    the procedure. There is a very rare chance that the testicle itself will be damaged during the

    Will the microscopic testicular sperm extraction procedure need to be repeated?

    When possible, any extra sperm retrieved during testicular sperm extraction should be frozen to
    preserve the sperm and avoid further procedures. Although freezing the sperm may have some negative effects, medical experts believe that the outcome is generally the same as using non-frozen sperm infertility procedures such as intracytoplasmic sperm injection (ICSI), a type of in vitro fertilization injecting one sperm directly into one
    egg. In vitro fertilization is a procedure in which sperm and an egg are fertilized outside of the
    body, in a laboratory. The fertilized egg is then placed in the female’s uterus.
    If the microTESE procedure needs to be repeated, it is usually best to wait six to 12 months.

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